Reporter

Part:BBa_K3267001:Design

Designed by: Yuma Kanazawa   Group: iGEM19_NYU_Shanghai   (2019-10-15)


Quorum-Sensing Receiver Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1015
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1661


Design Notes

A degradation tag is added to examine the temporal GFP expression.


Source

T. Tschirhart, E. Kim, R. McKay, H. Ueda, H.C. Wu, A.E. Pottash, A. Zargar, A. Negrete, J.Shiloach, G.F. Payne, W.E. Bentley. Electronic control of gene expression and cell behaviour in Escherichia coli through redox signalling. Nat Commun, 8 (2017), p. 14030

References